RNA purification and analysis / Douglas T Gjerde (2009)

Public ISBD Titre : RNA purification and analysis : sample preparation, extraction, chromatography Type de document : texte imprimé Auteurs : Douglas T Gjerde, Auteur ; Lee Hoang, Auteur ; David Hornby, Auteur Editeur : Weinheim : Wiley-vch Verlag Année de publication : 2009 Importance : XI, 195 p. Présentation : ill. Format : 25 cm ISBN/ISSN/EAN : 978-3-527-32116-2 Note générale : Index. Langues : Anglais (eng) Mots-clés : ARN -- Analyse RNA -- Analysis -- Laboratory manuals RNA -- Chromatography Index. décimale : 543.544 Analyse par chromatographie. Chromatographie Résumé : This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation. Note de contenu : Au sommaire: 1. RNA extraction, separation, and analysis 2. Biological and chemical RNA 3. RNA sepaation: substrates, functional groups, mechanisms, and control 4. RNA extraction and analysis 5. RNA chromatography 6. RNA chromatography separation and analysis 7. RNA structure-function probing RNA purification and analysis : sample preparation, extraction, chromatography [texte imprimé] / Douglas T Gjerde, Auteur ; Lee Hoang, Auteur ; David Hornby, Auteur . - Weinheim : Wiley-vch Verlag, 2009 . - XI, 195 p. : ill. ; 25 cm. ISBN : 978-3-527-32116-2 Index. Langues : Anglais (eng) Mots-clés : ARN -- Analyse RNA -- Analysis -- Laboratory manuals RNA -- Chromatography Index. décimale : 543.544 Analyse par chromatographie. Chromatographie Résumé : This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation. Note de contenu : Au sommaire: 1. RNA extraction, separation, and analysis 2. Biological and chemical RNA 3. RNA sepaation: substrates, functional groups, mechanisms, and control 4. RNA extraction and analysis 5. RNA chromatography 6. RNA chromatography separation and analysis 7. RNA structure-function probing

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Code-barres	Cote	Support	Localisation	Section	Disponibilité	Etat_Exemplaire
054353	543.544 GJE	Papier	Bibliothèque Centrale	Chimie	Disponible	Consultation sur place